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The NanoDrop 1000 Spectrophotometer measures 1 ul samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In addition, the NanoDrop 1000 Spectrophotometer has the capability to measure highly concentrated samples without dilution (50X higher concentration than the samples measured by a standard cuvette spectrophotometer).

UV/VIS spectrophotometry is simple for samples as small as 1 ul using the NanoDrop 1000 Spectrophotometer. The small sample requirement and ease of use make the NanoDrop 1000 Spectrophotometer ideally suited for measuring:

• Nucleic acid concentration and purity of nucleic acid samples up to 3700 ng/ul (dsDNA) without dilution
• Fluorescent dye labeling density of nucleic acid microarray samples
• Purified protein analysis (A280) up to 100 mg/ml (BSA)
• Expanded spectrum measurement and quantitation of fluorescent dye labeled proteins, conjugates, and metalloproteins
• Bradford Assay analysis of protein
• BCA Assay analysis of protein
• Lowry Assay analysis of protein
• Pierce Protein 660 nm Protein Assay
• Cell density measurements
• General UV-Vis spectrophotometry

Nanodrop 1000 ND-1000

    • Sample Size: 1 microliter
    • Path Length: 1 mm (with auto-ranging to 0.2 mm)
    • Light Source: Xenon flash lamp
    • Detector Type: 2048-element linear silicon CCD array
    • Wavelength Range: 220-750 nm
    • Wavelength Accuracy: 1 nm
    • Wavelength Resolution: 3 nm (FWHM at Hg 546 nm)
    • Absorbance Precision: 0.003 absorbance ( 1mm path)
    • Absorbance Accuracy: 2% (at 0.76 absorbance at 257 nm)
    • Absorbance Range: 0.02-75 (10 mm equivalent absorbance)
    • Detection Limit: 2 ng/microliter (dsDNA)
    • Maximum Concentration: 3700 ng/microliter (dsDNA)
    • Measurement Cycle Time: 10 seconds
    • Dimensions: 14 cm X 20 cm
    • Weight: 1.6 kg
    • Sample Pedestal Material of Construction: 303 stainless steel and quartz fiber
    • Operating Voltage: 12 Vdc
    • Operating Power Consumption: 6 W
    • Standby Power Consumption: 1.5 W Heating : 37 ± 0.5 °C
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